Solution Information | help | |
Enzyme: | 3-phosphoinositide-dependent protein kinase 1 | |
inhibitor: | BDBM17051 | |
substrate: | Serine/threonine-protein kinase AKT2 | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.2 | |
Temp. Prep.: | 22 | |
Comments: | n/a | |
Solute 1: | beta-glycerol phosphate | |
Concentration: | 18 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | MgOAc | |
Concentration: | 10 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | DTT | |
Concentration: | 0.7 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | ATP | |
Concentration: | 7.5 uM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | MOPS | |
Concentration: | 15 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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